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Quantitative real-time PCR in aDNA research

Bunce, M., Oskam, C.L. and Allentoft, M.E. (2012) Quantitative real-time PCR in aDNA research. Methods in molecular biology, 840 . pp. 121-132.

Link to Published Version: http://dx.doi.org/10.1007/978-1-61779-516-9_16
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Abstract

Quantitative real-time PCR (qPCR) is a technique that is widely used in the field of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing efficient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates.

Publication Type: Journal Article
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Publisher: Humana Press, Inc.
Copyright: © 2012 Springer Science+Business Media, LLC.
URI: http://researchrepository.murdoch.edu.au/id/eprint/7068
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