Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease
Abbiss, H., Maker, G.L., Gummer, J., Sharman, M., Phillips, J.K., Boyce, M. and Trengove, R.D. (2012) Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease. Nephrology, 17 (2). pp. 104-110.
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Aim: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. Methods: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. Results: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. Conclusion: The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Separation Science and Metabolomics Laboratory
School of Veterinary and Biomedical Sciences
|Copyright:||© Asian Pacific Society of Nephrology|
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