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PCR-sequence-specific primer typing of HLA class I and class II alleles

Bunce, M. (2003) PCR-sequence-specific primer typing of HLA class I and class II alleles. Methods in molecular biology, 210 . pp. 143-171.

Link to Published Version: http://doi.org/10.3138/jvme.33.1.93
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Abstract

The use of molecular typing methods for defining human leukocyte antigen (HLA) Class I and Class II alleles is now commonplace. Molecular methods offer a greater accuracy than traditional serological methods (1–5) which is reflected in many disease and transplantation studies (6–9). This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10,11) . PCR-SSP is also variably known as allele-specific PCR or amplification refractory mutation system (ARMS).

Publication Type: Journal Article
Publisher: Humana Press, Inc.
URI: http://researchrepository.murdoch.edu.au/id/eprint/5908
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