Molecular cloning of two new HLA-C alleles: Cw*1801 and Cw*0706

Vilches, C., Bunce, M., Sanz, L., Pablo, R., Puente, S. and Kreisler, M. (1996) Molecular cloning of two new HLA-C alleles: Cw*1801 and Cw*0706. Tissue Antigens, 48 (6). pp. 698-702.

Abstract

Nucleotide sequence analysis of the HLA-C alleles of the GB92 cell line, heterozygous for B*8101 and B*4407, revealed the existence of two new allelic variants: Cw*1801 and Cw*0706. The former allele, initially detected as a PCR-SSP variant, displays a hybrid aspect, sharing sequence motifs with Cw*07 at exons 1 and 2, and with'Cw*04 at distal exons. In serological assays, Cw*1801 is only recognized by some cross-reactive sera. Cw*0706 shows a primary structure closely related to previously known Cw7 alleles, but carries new sequence motifs at its 3′-end. Preliminary data indicate that Cw*1801 is associated to B*8101 and that Cw*0706, B*4407 could account for a part of the Cw7, B44 haplotypes observed in African populations.