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Genomic characterisation of Bettongia penicillata papillomavirus type 1

Bennett, Mark (2011) Genomic characterisation of Bettongia penicillata papillomavirus type 1. Masters by Research thesis, Murdoch University.

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      Abstract

      The first fully sequenced papillomavirus from a marsupial host, Bettongia penicillata papillomavirus type 1 (BpPV1), was detected in facial papillomas from an adult male woylie (Bettongia penicillata ogilbyi). Woylies are critically endangered Australian diprotodont marsupials that now occur only in parts of south-west Western Australia and on a few islands off the coast of South Australia. The gross and microscopic pathology was typical of papillomavirus infections in a wide range of animal species. Papillomavirus L1 protein was demonstrated using indirect immunohistochemistry within keratinocyte nuclei, as was BpPV1 DNA using in situ hybridization. The circular, double-stranded DNA genome contained 7737 base pairs and encoded 7 open reading frames: E6, E7, E1, E2, E4, L2 and L1 in typical papillomavirus conformation. Phylogenetic analysis revealed that BpPV1 is a close-to-root papillomavirus within the beta-gamma-pi-xi (β + γ + π + ξ) supertaxon of the family Papillomaviridae. It appears to be most closely related to Erinaceus europaeus papillomavirus type 1 – a papillomavirus detected in the European hedgehog. A phylogenetic tree constructed using the L1 and L2 open reading frames of BpPV1, 62 other papillomavirus types and the bandicoot papillomatosis-carcinomatosis viruses types 1 and 2 (BPCVs), revealed possible common ancestry between the late protein-encoding open reading frames of the BPCVs and BpPV1. This result is important for determining the likely evolutionary history of the BPCVs, and lends credence to the hypothesis that the BPCVs arose due to a recombination event between an ancient papillomavirus and an ancient polyomavirus, several tens of millions of years before present.

      Publication Type: Thesis (Masters by Research)
      Murdoch Affiliation: School of Veterinary and Biomedical Sciences
      Supervisor: Nicholls, Philip, O'Hara, Amanda, Warren, Kristin and Mills, Jennifer
      URI: http://researchrepository.murdoch.edu.au/id/eprint/5810
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