Catalog Home Page

In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville)

Bennett, M.D., Woolford, L., O'Hara, A.J., Warren, K.S. and Nicholls, P.K. (2008) In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville). Journal of General Virology, 89 (2). pp. 419-423.

[img]
PDF - Authors' Version
Download (372kB)
Link to Published Version: http://dx.doi.org/10.1099/vir.0.83455-0
*Subscription may be required

Abstract

The western barred bandicoot (Perameles bougainville) is an endangered Australian marsupial species in which a papillomatosis and carcinomatosis syndrome occurs. Bandicoot papillomatosis carcinomatosis virus type 1 (BPCV1) is associated with the lesions of this progressively debilitating syndrome. Five digoxigenin-labelled DNA probes were generated for in situ hybridization (ISH) and the technique was optimized and performed on formalin-fixed paraffin-embedded (FFPE) biopsies. Staining of keratinoicyte and sebocyte, nuclei within lesions was achieved with all five probes. The sensitivity of ISH (76.9 %) surpassed that of PCR (30.8%) for FFPE samples. The sensitivity of ISH varied from 81 % (papillomas) and 70% (carcinoma in situ) to 29% (squamous cell carcinomas). The specificity of the test was confirmed using an irrelevant probe and papillomas from other species. These results strengthen the association between BPCV1 and the western barred bandicoot papillomatosis and carcinomatosis syndrome and give insight into the biology of the virus-host interaction.

Publication Type: Journal Article
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Publisher: Society for General Microbiology
Copyright: © 2008 SGM.
URI: http://researchrepository.murdoch.edu.au/id/eprint/539
Item Control Page Item Control Page

Downloads

Downloads per month over past year