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Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis

Siripattanapipong, S., Leelayoova, S., Mungthin, M., Thompson, R.C.A., Boontanom, P., Saksirisamphant, W. and Tan-ariya, P. (2011) Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis. Southeast Asian Journal of Tropical Medicine and Public Health, 42 (4). pp. 764-771.

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    Abstract

    Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), beta-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-alpha) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from Gen Bank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Veterinary and Biomedical Sciences
    Publisher: SEAMEO Regional Tropical Medicine and Public Health Network
    Publishers Website: http://www.tm.mahidol.ac.th/seameo/publication.htm
    URI: http://researchrepository.murdoch.edu.au/id/eprint/5036
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