Characterisation and cross-species utility of 20 microsatellite markers for population and forensic applications in the endangered Carnaby’s Black-cockatoo, Calyptorhynchus latirostris

White, N.E., Mawson, P.R., Dawson, R., Bunce, M.A. and Spencer, P.B.S. (2009) Characterisation and cross-species utility of 20 microsatellite markers for population and forensic applications in the endangered Carnaby’s Black-cockatoo, Calyptorhynchus latirostris. Conservation Genetics Resources, 1 (1). pp. 341-345.

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Abstract

We characterise 20 microsatellite loci identified from the endangered Carnaby’s Black-cockatoo (Calyptorhynchus latirostris). The primers were tested across 40 individuals from the southwest of Western Australia and displayed between 4 and 11 alleles per locus with expected heterozygosities ranging from 53 to 87% and exclusion probabilities of C0.999. These loci will be useful in population genetic studies to facilitate conservation management decisions in addition to wildlife enforcement applications for the endangered Carnaby’s Black-cockatoo. We also tested the markers in 12 high profile and smuggled species from five genera, Cacatua, Callocephalon, Calyptorhynchus, Nymphicus and Probosciger. These species detected between 2 and 19 alleles per locus with 50–100% amplification success.