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Extraction and amplification of DNA from the dried rostra of sawfishes (Pristidae) for applications in conservation genetics

Phillips, N., Chaplin, J., Morgan, D.L. and Peverell, S. (2009) Extraction and amplification of DNA from the dried rostra of sawfishes (Pristidae) for applications in conservation genetics. Pacific Conservation Biology, 15 (2). pp. 128-134.

Abstract

DNA was obtained from the skin tissue of a high proportion (198/236) of dry rostra collected during the past -30 years from the sawfishes Pristis microdon, P. clavata, P. zijsron and Anoxypristis cuspidate.DNA obtained from the dry rostra was usually either non-degraded, or only partly degraded, and was of a quality whereby a 465-468-bp portion of the mtDNA 12S rRNA gene could be PCR-amplified. These findings demonstrate that it is possible to use the dry rostra, which are generally readily available, as a source of DNA in conservation genetic studies of sawfishes. Such studies are needed for the development of effective conservation strategies for these critically endangered species, but would otherwise be greatly constrained by the difficulties of obtaining samples from sufficient numbers of living sawfishes

Publication Type: Journal Article
Murdoch Affiliation: Centre for Fish and Fisheries Research
Publisher: Surrey Beatty & Sons
Copyright: 2009 Surrey Beatty & Sons
Publishers Website: http://pcb.murdoch.edu.au/
URI: http://researchrepository.murdoch.edu.au/id/eprint/3948
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