Aligning a new reference genetic map of lupinus angustifolius with the genome sequence of the model legume, Lotus japonicus
Nelson, M.N., Moolhuijzen, P.M., Boersma, J.G., Chudy, M., Lesniewska, K., Bellgard, M., Oliver, R.P., Swiecicki, W., Wolko, B., Cowling, W.A. and Ellwood, S.R. (2010) Aligning a new reference genetic map of lupinus angustifolius with the genome sequence of the model legume, Lotus japonicus. DNA Research, 17 (2). pp. 73-83.
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We have developed a dense reference genetic map of Lupinus angustifolius (2n = 40) based on a set of 106 publicly available recombinant inbred lines derived from a cross between domesticated and wild parental lines. The map comprised 1090 loci in 20 linkage groups and three small clusters, drawing together data from several previous mapping publications plus almost 200 new markers, of which 63 were gene-based markers. A total of 171 mainly gene-based, sequence-tagged site loci served as bridging points for comparing the Lu. angustifolius genome with the genome sequence of the model legume, Lotus japonicus via BLASTn homology searching. Comparative analysis indicated that the genomes of Lu. angustifolius and Lo. japonicus are highly diverged structurally but with significant regions of conserved synteny including the region of the Lu. angustifolius genome containing the pod-shatter resistance gene, lentus. We discuss the potential of synteny analysis for identifying candidate genes for domestication traits in Lu. angustifolius and in improving our understanding of Fabaceae genome evolution.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Centre for Comparative Genomics|
Western Australian State Agricultural Biotechnology Centre
|Publisher:||Oxford University Press|
|Copyright:||© 2010 The Author.|
|Notes:||This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.|
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