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Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system

Borowski, H., Thompson, R.C.A., Armstrong, T. and Clode, P.L. (2010) Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system. Parasitology, 137 (01). pp. 13-26.

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    Link to Published Version: http://dx.doi.org/10.1017/S0031182009990837
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    Abstract

    Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages of C. parvum and their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect of C. parvum on host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Veterinary and Biomedical Sciences
    Publisher: Cambridge University Press
    Copyright: © 2009 Cambridge University Press
    URI: http://researchrepository.murdoch.edu.au/id/eprint/3766
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