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Capturing genetic information using non-target species markers in a species that has undergone a population crash

Pacioni, C. and Spencer, P.B.S. (2010) Capturing genetic information using non-target species markers in a species that has undergone a population crash. Australian Mammalogy, 32 (1). pp. 33-38.

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    Link to Published Version: http://dx.doi.org/10.1071/AM09018
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    Abstract

    Species conservation has relied on the enormous potential of information that arises from field, laboratory and other tools. When using molecular-based tools, the technology involves a considerable effort to develop, both in resources and time. A long-held practice has been to utilise pre-existing primers developed for other closely related species to evaluate conservation questions. In this study, we present a practical approach on how to utilise pre-existing microsatellite markers in bettong and potoroo species. This information is relevant before, during and after a species crash and the approach we describe could be particularly appropriate when there is an immediate need to retrieve a knowledge-base in order to support management decisions. We determined that cross-species amplification success of microsatellite markers is inversely related to evolutionary distance of the source species although their polymorphism is not. A 'priority-list' of potential markers for potoroids is given for future conservation genetic studies.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Biological Sciences and Biotechnology
    School of Veterinary and Biomedical Sciences
    Publisher: Australian Mammal Society Inc.
    Copyright: © Australian Mammal Society 2010.
    URI: http://researchrepository.murdoch.edu.au/id/eprint/3745
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