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Comparison of baiting and molecular methods for the detection of Phytophthora cinnamomi form soil samples from south Western Australia

Anderson, N.M., Hardy, G.E.St.J. and O'Brien, P.A. (2005) Comparison of baiting and molecular methods for the detection of Phytophthora cinnamomi form soil samples from south Western Australia. In: 15th Australasian Plant Pathology Society Conference, 26-29 September 2005 , Geelong, Vic..

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    Abstract

    Phytophthora cinnamomi has been responsible for widespread destruction of native forests and heath lands throughout southern Australia. Baiting has been the primary method for detecting p, cinnamomi from soil samples (I). However, it is limited by the common occurrence of false negatives and the inability to detect dormant spores within the soil (2). The introduction of molecular based detection methods offer improved sensitivity and specificity for P. cinnamomi detection (3). PCR amplification is central to various methods of molecular detection including PCR-RFLP and microarray analysis. Several previous studies have compared the theoretical sensitivity of PCR and baiting of zoospore and chlamydospore inoculations without assessing naturally infested soils (4). This study compares baiting to primary and nested PCR detection of P. cinnamomi from naturally infested soils.

    Publication Type: Conference Item
    Murdoch Affiliation: Centre for Phytophthora Science and Management
    URI: http://researchrepository.murdoch.edu.au/id/eprint/3532
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