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Distribution of Phytophthora cinnamomi across disease sites in Western Australia

Williams, N., Hardy, G.E.St.J. and O'Brien, P.A. (2008) Distribution of Phytophthora cinnamomi across disease sites in Western Australia. In: ICPP 2008 9th International Congress of Plant Pathology, 24 - 29 August, Torino, Italy,.

Abstract

Phytophthora cinnamomi is currently causing disease in a wide range of native Australian plant species. We have carried out a systematic survey of the distribution of the pathogen at seven disease sites in Western Australia using both PCR and baiting to detect the pathogen in soil samples. A nested PCR assay based on ITS sequences was developed for detection of the pathogen in soil. This can detect 1 pg of P. cinnamomi DNA. When baiting was used for detection, the number of positive samples ranged from 1–10%. Analysis of material from the selective plates by the PCR assay significantly increased the number of positive samples. When DNA extracted directly from soil was analysed by nested PCR, over 90% of the samples were positive. This was not due to cross-contamination between samples, as stringent quality control was in place to detect such cross-contamination.

Publication Type: Conference Item
Murdoch Affiliation: Centre for Phytophthora Science and Management
URI: http://researchrepository.murdoch.edu.au/id/eprint/3530
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