Characterisation of the regulatory circuit required for the acid induction of the adaptive acid tolerance response Protein LPIA in Sinorhizobium Medicae WSM419
Tian, R., Tiwari, R., Bräu, L. and Reeve, W. (2009) Characterisation of the regulatory circuit required for the acid induction of the adaptive acid tolerance response Protein LPIA in Sinorhizobium Medicae WSM419. In: The 16th International Congress of Nitrogen Fixation, 14 - 19 June, Big Sky, Montana.
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The acid tolerance response of Sinorhizobium medicae WSM419 enables cell adaptation to lethal acid after cell exposure to mild acidity. The expression of the LPIA (low pH induced gene A) gene is critical for this response and is add-activated at least 20-fold in the mild acidic conditions. The expression of this gene is specifically induced by acidity and not by any other stress. We have previously shown that full acid-induction of lpiA requires functional FsrR (fused sensor-regulator) (Reeve et al 2006). However, even in the absence of FsrR, there is still a residual6-fold acid-induction of tpiA implicating other proteins in the regulation process. Other genes have been suspected to be involved including tcsA (two component sensor) tcrA (two component regulator), acvB (acid virulence protein B) and rpoN (RNA polymerase N-metabolism).
|Publication Type:||Conference Item|
|Murdoch Affiliation:||Centre for Rhizobium Studies|
School of Biological Sciences and Biotechnology
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