Broth recycling to reduce process noise resulting from concentrated substrate addition in fed-batch cultivation of Escherichia coli
Johnston, W.A. and Cooney, M.J. (2003) Broth recycling to reduce process noise resulting from concentrated substrate addition in fed-batch cultivation of Escherichia coli. Biotechnology Progress, 19 (2). pp. 659-661.
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In this work feed hardware for fed-batch cultivation is presented (broth recycle feed injection system or BRFIS). BRFIS proved superior to conventional submerged or dripped feed systems in reducing dissolved oxygen (DO) oscillations during Escherichia coli fed-batch cultivation (5 min coefficient of variation of 0.7% for BRFIS as compared to 26% or greater for conventional feeding hardware in a 2 L test reactor). Hence, BRFIS is useful for fed-batch cultivation systems where the DO signal is used in measurement or control.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Engineering|
|Publisher:||John Wiley and Sons Ltd|
|Copyright:||© 2003 American Institute of Chemical Engineers (AIChE)|
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