An alternative nitrogenase is not expressed in molybdenum-deficient legume root nodules
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Three legume root nodule bacteria systems (Medicago polymorpha L. ‐Rhizobium meliloti, Ornithopus sativus Brot. ‐Bradyrhizobium lupini and Trifolium subterraneum L.‐ Rhizobium leguminosarum by. trifolii) were grown in solution culture under conditions likely to lead to the production of alternative nitrogenases (molybdenum‐deficient, or molybdenum‐deficient but supplemented with vanadium). Addition of 1 μM molybdenum produced significant responses in both nodule and top weights while 2 μM vanadium did not. Ethane, which is produced as well as ethylene when acetylene is reduced by vanadium nitrogenase or nitrogenase‐3 from Azotobacter, was not found in significant amounts during assays of acetylene reduction in either molybdenum‐deficient or molybdenum‐deficient, vanadium‐supplemented treatments, suggesting that no non‐molybdenum nitrogenase was produced by these root nodule bacteria.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological and Environmental Sciences|
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