Effect of harvest time on fungal community development on Eucalyptus globulus coppice stumps
Tovar, F.J., Burgess, T., Robinson, R.M. and Hardy, G.E.St.J. (2008) Effect of harvest time on fungal community development on Eucalyptus globulus coppice stumps. In: ICPP 2008 9th International Congress of Plant Pathology, 24 - 29 August, Torino, Italy,.
In Western Australia, reports of stump rot and wind-throw losses in second rotation Eucalyptus globulus coppice plantations led to an investigation of the possible negative impacts of fungi colonising stumps. Surveys showed that a variety of fungi colonise stumps, but no geographic trends were apparent. As plantations are harvested at different times of the year, it was hypothesized that differences between plantations may be due to harvest times coinciding with different fungal fruiting and dispersal times. This was tested by harvesting fifteen trees at each season (spring-Oct 2006, summer-Jan 2007, autumn-May 2007, and winter-July 2007). Five of the trees were assigned as controls and only had wood samples analysed for the presence of fungi at the start and end of the experiment. The remaining ten trees were sampled for fungi at the time of harvest and 1, 4, 8 and 12 months after harvest. The aim was to observe seasonal differences in fungal community development within the outer sapwood and the inner discoloured central core. Presence of fungi was determined using molecular and classical cultural techniques. In the classical approach wood samples extracted from the stumps were plated on four different agar media to select for as wide variety of fungi as possible. The molecular approach used RFLP analysis of genes encoding rRNA to detect the presence of any fungi in the samples. Results from this investigation will be presented and discussed.
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|Murdoch Affiliation:||School of Biological Sciences and Biotechnology|
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