Optimisation of a reporter cell line for assessing neutralising antibody titres to hepatitis C virus
Van Trigt, J., Clark, H., Watson, M. and Allan, J. (2013) Optimisation of a reporter cell line for assessing neutralising antibody titres to hepatitis C virus. In: 23rd Annual Combined Biological Sciences Meeting (CBSM) 2013, 30 August 2013, University of Western Australia, Perth.
Neutralising antibodies to hepatitis C virus (HCV) are studied to characterise the determinants of HCV virulence and for vaccine and immunotherapy development. To discover useful antibodies, patients are screened for HCV neutralising activity by incubating their serum with a lab strain of HCV, and incubating this with susceptible cells. Days later the cultures are stained to reveal infected cells and the amount of infecting virus is expressed as focus forming units (FFU). This method is expensive and time-consuming. The Huh7-J20 reporter cell line, provided by Dr A Patel, may provide a cheaper and faster alternative. When this line is infected with HCV, the NS3/4A viral protease cleaves a reporter protein, releasing secreted alkaline phosphatase (SEAP) into the culture supernatant, which can be measured by spectrophotometry.
|Publication Type:||Conference Item|
|Murdoch Affiliation:||Institute for Immunology and Infectious Diseases|
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