Polymerase chain reaction targetting the nox gene for identification of Serpulina intermedia in pigs
Atyeo, R.F., Suriyaarachchi, D.S., Stanton, T.B., Jenson, N.S. and Hampson, D.J. (1998) Polymerase chain reaction targetting the nox gene for identification of Serpulina intermedia in pigs. In: 15th International Pig Veterinary Society Congress, 5 - 9 July, Birmingham, UK p. 129.
Serpulina intermedia (formerly "S. intemedius") is a recently named species of weakly B-haemolytic, indole positive anaerobic intestinal spirochaete (7). In diagnostic laboratories it can be easily confused with the strongly β-haemolytic, indole positive S. hyodysenteriae (the agent of swine dysentery), or one of several other weakly β-haemolytic non-pathogenic Serpulina species. S. intermedia is considered to be a pathogen of poultry (5), however evidence for its pathogenic potential in pigs remains equivocal (3). Strains of what appear to be S. intermedia have been isolated from pigs with diarrhoea in Poland (1) and Sweden (2), but in experimental studies infection of conventional pigs with S. intermedia type strain PWS/ A did not result in disease (4). The purpose of this study was to develop a polymerase chain reaction (PCR) test for the identification of S. intermedia strains using sequence information derived from the NADH oxidase (nox) gene of the spirochaete. NADH oxidase has been detected in every Serpulina strain tested and thus may be an identifying trait for the genus (7).
|Publication Type:||Conference Paper|
|Murdoch Affiliation:||School of Veterinary and Biomedical Sciences|
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