Improved DNA fingerprints for macropods generated with a (CA)n-containing satellite fragment derived from the allied rock wallaby, Petrogale assimilis
Odorico, D.M., Spencer, P.B.S. and Miller, D.J. (1992) Improved DNA fingerprints for macropods generated with a (CA)n-containing satellite fragment derived from the allied rock wallaby, Petrogale assimilis. Wildlife Research, 19 . pp. 397-404.
Limited success with 'standard' probes led us to seek more informative polymorphic sequences as candidate probes for multilocus DNA fingerprinting of macropods. We describe the construction and evaluation of a (CA)n-containing clone from the allied rock-wallaby, Petrogale assimills. This probe (B 10) was both more informative and more sensitive than bacteriophage M13 for P. assimilis and Thylogale stigmatica. For P. assimilis and T. stigmatica the B1O probe detected on average 3 times and 10 times more Hinf I bands (longer than 3 kb), respectively, than did M13, and exposure times were typically an order of magnitude shorter with B1O. The preliminary data presented imply that for P. assimills this probe should permit parentage to be assigned with confidence from single-enzyme digestion data even in populations of 50—100 individuals. The probe also generates complex restriction patterns for a range of other macropods.
|Publication Type:||Journal Article|
|Item Control Page|