Microsatellites from Clarias batrachus and their polymorphism in seven additional catfish species
Yue, G-H, Kovács, B. and Orbán, L. (2003) Microsatellites from Clarias batrachus and their polymorphism in seven additional catfish species. Molecular Ecology Notes, 3 (3). pp. 465-468.
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We isolated 18 novel microsatellite loci from the walking catfish (Clarias batrachus), and examined their cross-amplification in seven additional catfish species from three families. Sixteen of the 18 microsatellites were polymorphic in the source species (allele number: 2–10/locus and expected heterozygosity: 0.30–0.87). Moreover, nine of these 18 primer pairs cross-amplified specific and polymorphic products from the genome of at least six of the seven other catfish species tested. However, the success rate of cross-species amplification varied from locus to locus, indicating that cross-species amplification of microsatellites is locus-dependent.
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