A simple and rapid method to determine vegetative compatibility groups in fungi
Burgess, T., Bihon, W., Wingfield, M.J. and Wingfield, B.D. (2009) A simple and rapid method to determine vegetative compatibility groups in fungi. Inoculum: Newsletter of the Mycological Society of America, 60 (6). pp. 1-2.
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Vegetative compatibility in fungi reflects phenotypic differences (or similarity) among individuals representing the population of a species (Leslie 1993). Thus, individuals (genotypes) of a fungal species having the same heterokaryon (het) or vegetative incompatibility (vic) loci can fuse to form a heterokaryon (Glass et al 2000). Fungal isolates that form stable heterokaryons are then considered to belong to same vegetative compatible group (VCG). In contrast, isolates that are different at one or some or more of these loci will not anastomose. Rather, programmed cell death or apoptosis occurs in the mycelial cells that are in contact with an isolate representing a different VCG (Anagnostakis 1987, Leslie 1993). In the case of fungi which have coloured or dark mycelium in culture (such as most Botryosphaeriaceae and Cryphonectriaceae) failure to anastomose is observed as a thick barrage line between the two different isolates. For such species, tests in Petri dishes make it relatively easy to determine the VCG’s for a population of isolates and this provides a robust view of population diversity (Burgess et al 2001, van Heerden and Wingfield 2001). For fungi with light coloured mycelium, for example species of Fusarium, barrage zones between isolates having different VCG’s are difficult to discern. In such cases, it is necessary to produce nit mutants to define the individuals in culture (Klittich and Leslie 1988, Swift 2002).
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological Sciences and Biotechnology|
|Publisher:||The Mycological Society of America|
|Notes:||Supplement to Mycologia Vol. 60(6) December 2009|
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