Neuronal cell culture metabolomics to study illicit drug use
A Metabolomics approach has been used to determine the response of neuronal cells to the illicit drug methamphetamine and the cocktail with caffeine. Neuronal cells have been cultured and then exposed to chronic levels of methamphetamine and the commonly used cocktail, methamphetamine with caffeine (YABA). The cells were harvested after the exposure period and the cells and media analysed using GC-MS based Metabolomics. Changes in metabolite regulation in the exposed cells and their media were determined by comparison of the metabolite levels in control cells and media. Up-regulated and down-regulated metabolites were identified and mapped to biochemical pathways to investigate the pathway modification caused by the drug and dug cocktail. The pathway redefinition has been compared with literature models of methamphetamine neuronal damage.
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