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Quantitative fungal metabolomics using GS/MS/MS-quantification of the major sources of variance from GC/MS studies

Trengove, R., Peebles, B., Rawlinson, C., Gummer, J., Maker, G.L., Tan, K., Krill, C., Roussety, K., Cruz, C.A., Solomon, P., Oliver, R., Muntean, F., Schachterle, S., Kellogg, C. and Jeanville, P. (2011) Quantitative fungal metabolomics using GS/MS/MS-quantification of the major sources of variance from GC/MS studies. In: Seventh International Conference of the Metabolomics Society, 27 - 30 June, Cairns, Qld, Australia.

Abstract

Stagonospora nodorum is a serious fungal pathogen of wheat and results in annual production losses of $108 million in Australia. Comparative Metabolomics studies have been undertaken between the wild type Stagonospora nodorum and mutant strains that are defective in cyclic AMP signalling, exhibit abnormal development and reduction in pathogenicity, using single quadrupole GC/MS with a split/splitless inlet. These comparative studies have identified metabolites that were differentially abundant between the wild type and mutants and studies are now in progress to c these differences with the compromised phenotype of the mutant. We have developed a targeted tandem mass spectrometry method using triple quadrupole GC/MS to quantify the abundances of the fungal specific metabolites for the wild type and the mutants. The triple quadrupole instrument has been modified to include a PTV injection system and has collected both MS/MS and full scan data to correlate with the existing single quadrupole GC/MS results. In this initial methodology MRMs for almost 50 metabolites have been added to the method. The initial quantitative studies were undertaken on in vitro cultures of the strains and the next step to assess the biological significance of these changes is to determine the abundance of these metabolites during infection of the plant.

Publication Type: Conference Item
Murdoch Affiliation: Separation Science and Metabolomics Laboratory
URI: http://researchrepository.murdoch.edu.au/id/eprint/29295
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