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Analysis of inter-laboratory metabolomics experiments

Nahid, A., Olshansky, M., D'Souza, D., Dias, D., Rawlinson, C., Silva, C., Maker, G.L., Tull, D., Roessner, U., McConville, M.J., Bacic, A., Trengove, R.D. and Speed, T.P. (2011) Analysis of inter-laboratory metabolomics experiments. In: Seventh International Conference of the Metabolomics Society, 27 - 30 June, Cairns, Qld, Australia.

Abstract

Metabolomics Australia is developing standardised retention time locked GC-MS analyses methods that can be used in a laboratory independent manner and derive the same result. Achieving this level of reproducibility would allow for analysis of samples from large projects at multiple sites potentially increasing throughput substantially. To validate our methods we have prepared a standard mix containing ~40 known metabolites (mm). We have also prepared an additional version of this mix, where a selection of metabolites is spiked at a significantly higher abundance (mm+). Replicates (6-8) of the unspiked (mm) and spiked (mm+) metabolite mix were run on four different GC-MS machines at three laboratories using two GC-MS methods differing in temperature gradient. Target ion areas for the metabolites in the mix were extracted using Analyser Pro software (SpectralWorks) and presented as a matrix for statistical analysis. A linear model with an interaction term(s) is presented here to analyse and interpret these inter-laboratory metabolomics measurements. This model will give us a basis to build more complex models where different groups/types of samples from a single study are processed at different laboratories. This work is funded by Metabolomics Australia

Publication Type: Conference Item
Murdoch Affiliation: Separation Science and Metabolomics Laboratory
School of Pharmacy
URI: http://researchrepository.murdoch.edu.au/id/eprint/29285
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