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Metabolomics study of the wheat defensome under pathogen challenge - interlaboratory GC/MS method development

Rawlinson, C., Dias, D., Nahid, A., Risk, J., Rybak, K., Oliver, R., Solomon, P., Maker, G.L., Tan, K., D'Souza, D., Trengove, R.D. and Roessner, U. (2011) Metabolomics study of the wheat defensome under pathogen challenge - interlaboratory GC/MS method development. In: Seventh International Conference of the Metabolomics Society, 27 - 30 June, Cairns, Qld, Australia.


Pathogens are a major threat to Australian grain production, causing significant production losses in Australia every year. A 2008 review by Murray and Brennan estimated that pathogens cost the Australian wheat industry $913 million per annum, making up 19.5% of the current crop value. Without current management practices these losses would be far greater.

Bioplatforms Australia has facilitated the generation of reference data infrastructure for wheat and wheat pathogens by co-funding Australia's contribution to the International Wheat Genome Sequencing Consortium (IWGSC) and the generation of reference data sets from the measurement of the wheat defensome under pathogen attack using Metabolomics, Proteomics, Transcriptomics and Genomics.

The Metabolomics analysis is being undertaken at multiple nodes of Metabolomics Australia, using GC/MS, LC/MS, CE/MS and NMR platforms for analysis. The GC/MS extraction and instrument method has been validated using unchallenged Chinese Spring samples across nodes of Metabolomics Australia using quadrupole GC/MS and GCxGC TOF MS platforms.

Wheat pathology laboratories across the country are challenging wheat with the major pathogens causing production losses and these samples extracted in house for analysis by Metabolomics Australia nodes to determine the wheat defensome compounds activated by each pathogen type. Data for the wheat defensome challenged by a key pathogen from the Western region of Australia will be discussed

Publication Type: Conference Item
Murdoch Affiliation: Separation Science and Metabolomics Laboratory
School of Pharmacy
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