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Molecular Biology: RNA polymerase II is required for RNAi-dependent heterochromatin assembly

Kato, H., Goto, D.B., Martienssen, R.A., Urano, T., Furukawa, K. and Murakami, Y. (2005) Molecular Biology: RNA polymerase II is required for RNAi-dependent heterochromatin assembly. Science, 309 (5733). pp. 467-469.

Link to Published Version: http://dx.doi.org/10.1126/science.1114955
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Abstract

In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.

Publication Type: Journal Article
Publisher: American Association for the Advancement of Science
URI: http://researchrepository.murdoch.edu.au/id/eprint/28437
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