Catalog Home Page

Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases

Wheeler, M., Chardon, A., Goubet, A., Morihiro, K., Tsan, S.Y., Edwards, S.L., Kodama, T., Obika, S. and Veedu, R.N. (2012) Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases. Chemical Communications, 48 (89). pp. 11020-11022.

[img]
Preview
PDF - Published Version
Download (1MB)
Link to Published Version: http://dx.doi.org/10.1039/C2CC36464F
*Subscription may be required

Abstract

Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.

Publication Type: Journal Article
Publisher: Royal Society of Chemistry
Copyright: 2012 The Royal Society of Chemistry
URI: http://researchrepository.murdoch.edu.au/id/eprint/27406
Item Control Page Item Control Page

Downloads

Downloads per month over past year