Targeting VEGF with LNA-stabilized G-rich oligonucleotide for efficient breast cancer inhibition
Edwards, S.L., Poongavanam, V., Kanwar, J.R., Roy, K., Hillman, K.M., Prasad, N., Leth-Larsen, R., Petersen, M., Marušič, M., Plavec, J., Wengel, J. and Veedu, R.N. (2015) Targeting VEGF with LNA-stabilized G-rich oligonucleotide for efficient breast cancer inhibition. Chemical Communications, 51 (46). pp. 9499-9502.
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In this study, we investigated the efficacy of an LNA (locked nucleic acid)-modified DNA aptamer named RNV66 targeting VEGF against various breast cancer cell lines. Our results demonstrate that RNV66 efficiently inhibits breast cancer cell proliferation both in vitro and in vivo. Introduction of LNA nucleotides were crucial for higher efficacy. Furthermore, the binding interaction of RNV66 with VEGF was investigated using molecular dynamic simulations leading to the first computational model of the LNA aptamer–VEGF complex blocking its interaction with VEGF-receptor.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Centre for Comparative Genomics|
|Publisher:||Royal Society of Chemistry|
|Copyright:||© 2015 The Royal Society of Chemistry|
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