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A simple method for cloning Giardia duodenalis from cultures and fecal samples

Binz, N., Thompson, R.C.A., Meloni, B.P. and Lymbery, A.J. (1991) A simple method for cloning Giardia duodenalis from cultures and fecal samples. Journal of Parasitology, 77 (4). pp. 627-631.

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    Abstract

    Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Veterinary Studies
    Publisher: American Society of Parasitologists
    URI: http://researchrepository.murdoch.edu.au/id/eprint/2540
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