Evidence for alternate Pax7 transcripts in skeletal muscle, brain and other organs of adult mice
Ziman, M., Fletcher, S. and Kay, P. (1997) Evidence for alternate Pax7 transcripts in skeletal muscle, brain and other organs of adult mice. In: XIII International Congress of Neuropathology, 7 - 12 September 1997, Perth, Australia.
The development gene Pax7, is associated with formation of skeletal muscle and elements of the central nervous system in the developing embryo. Interestingly, in adult mice, rearrangements of Pax7 are associated with differences in the efficiency of skeletal muscle regrowth between mouse strains. We have therefore investigated the expression of Pax7 in various tissues from adult mice and as a result of these experiments, postulate that the different biological functions of Pax7 are effected by different transcripts of Pax7 Total RNA was isolated from various tissues of adult mice and RTPCR was performed using primers specific for the various regions of the gene encoding DNA binding domains, eg. the paired and homeodomains. Data is presented which demonstrates the presence of at least four different Pax7 transcripts. A full-length transcript similar to that of the published sequence has been identified in skeletal muscle, brain and some spleen cells of adult mice. A skeletal muscle specific full-length Pax7 transcript including a hexanucleotide insertion in the coding region of the paired box has also been identified in adult mice A further full-length transcript in which approximately 10 bp have been deleted in the homeodomain encoding region was found to be expressed in the brain of adult mice. A shorter Pax7 splice product comprising he paired domain encoding region only was found to be expressed in most adult tissues including the kidney heart and lung but not liver. These findings strongly suggest that Pax7 may have multiple biological functions not only during embryogenesis but also in the adult animal. One of these functions may be to control an alternate myogenic pathway operable in adult mice which is involved in regeneration of damaged skeletal muscle of the limbs.
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