Isolation and characterization of microsatellites in the woody shrub, Calothamnus quadrifidus (Myrtaceae)
Elliott, C.P. and Byrne, M. (2005) Isolation and characterization of microsatellites in the woody shrub, Calothamnus quadrifidus (Myrtaceae). Molecular Ecology Notes, 5 (4). pp. 757-759.
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Microsatellite loci were developed for genetic analysis of the bird pollinated woody shrub Calothamnus quadrifidus. A genomic library was constructed and screened with dinucleotide and trinucleotide repeat sequences. Ten dinucleotide microsatellite markers were developed, and polymorphism in a population of C. quadrifidus was investigated for six of these markers, which showed an average of 12.7 alleles per locus. Mendelian inheritance of the loci was confirmed through analysis of open pollinated progeny arrays of 10 plants. These loci will be used to study gene flow patterns between isolated populations of this species in southwest Western Australia.
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|Copyright:||© 2005 Blackwell Publishing Ltd.|
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