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PCR for identification of spirochaetes associated with intestinal spirochaetosis

Park, N.Y., Chung, C.Y., McLaren, A.J., Atyeo, R.F. and Hampson, D.J. (1994) PCR for identification of spirochaetes associated with intestinal spirochaetosis. In: Proceedings of the 13th International Pig Veterinary Society Congress, 26 - 30 June, Bangkok, Thailand p. 197.

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Weakly haemolytic intestinal spirochaetes, distinct from both Serpulina hyodysenteriae and Serpulina innocens, have been reported to cause a disease called intestinal spirochaetosis, or spirochaetal diarrhoea, in weaned pigs (1, 2, 3, 4) . Infected animals develop a sloppy mucoid diarrhoea and a reduction in growth rate. These spirochaetes are slightly more slender than S. innocens, and characteristically only have 4-6 axial flagellae ( 5). Strain P43/6/78, which was isolated from the first recorded case of porcine intestinal spirochaetosis ( 1), has been deposited with the American Type Culture Collection (as ATCC 51139) as the type strain of a possible new genus and species of spirochaete ( "Anguillina coli") ( 5). In the current study we have used a polymerase chain reaction (PCR) amplification of a section of the region encoding the 16s ribosomal RNA gene as a rapid and specific means of identifying these bacteria.

Publication Type: Conference Paper
Murdoch Affiliation: School of Veterinary Studies
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