Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus
Boulos, S. and Rood, J.I. (1986) Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus. FEMS Microbiology Letters, 33 (1). pp. 73-78.
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The fimbrial subunit gene from the benign type BBacteroides nodosus isolate AC/6 was cloned into theSphI site of the multicopy vector plasmid pUC19. FiveEscherichia coli recombinants that were positive in a colony immunoassay were shown, by Western transfer analysis, to produce an immunologically cross-reacting protein of identical molecular size to fimbrial subunits prepared fromB. nodosus AC/6. Restriction endonuclease analysis showed that 4 of the recombinant plasmids carried a 6.7 kbSphI fragment. Recloning experiments showed that the fimbrial subunit gene was located within a 2.5 kbEcoRI-SphI fragment and that there was aPstI site located within the structural gene or its regulatory region. These recombinant clones will prove useful for the construction of a multivalent recombinant vaccine for the control of ovine footrot.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary Studies|
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