Distribution of copper in the kidney of sheep after thiomolybdate administration
Chronic copper poisoning (CCP) is a well recognized harzard of sheep production (1). There is evidence that the incidence of CCP is increasing as more intensive methods of sheep production are adopted (2). In CCP, haemolysis is the most characteristic symptom and hence the disease has been categorized into 3 phases; pre-haemolytic, haemolytic and post-haemolytic (3, 4). Animals may develop several haemolytic crises despite the removal of the source of excess copper (Cu) at the time of the first haemolysis (5). Kidney damage occurs during haemolysis and persists into the post-haemolytic phase. Gooneratne et al. (6) have shown that thiomolybdate (TM) injections prevented the occurrence of the haemolytic crisis when given to Cu loaded sheep before haemolysis and were of value in treating sheep in which the haemolysis of CCP had already occurred. However, the kidneys of TM treated animals accumulated large amounts of Cu. The consequences of this were not known. The intracellular distribution of Cu in the kidney of either animals or humans has not been reported previously. The present investigation was carried out to determine the pattern of distribution of Cu within the subcellular fractions of the kidney in normal, TM treated and Cu loaded-TM treated sheep. Acid phosphatase (AP) activity was measured to identify the movement of lysosomes within the fractions as our previous studies on the kidney (7) indicated that there was an increase in lysosomes within the tubule cells of the kidney of experimental chronic copper poisoned sheep.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary Studies|
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