Effect of low oxygen on peroxidase activity in roots of clonal Eucalyptus marginata infected with Phytophthora cinnamomi
Burgess, T. and Hardy, G.E.St.J. (1996) Effect of low oxygen on peroxidase activity in roots of clonal Eucalyptus marginata infected with Phytophthora cinnamomi. In: American Phytopathological Society Annual Meeting, 27 - 31 July, Indianapolis, IN, USA.
Clones of Eucalyptus marginata, resistant to the pathogen Phytophthora cinnamomi, were grown in aeroponics chambers that could be sealed to allow the manipulation of oxygen levels. Roots grown under normal oxygen were compared with those that had been exposed to either 6 days of hypoxia (2 mg 02 L-1) or 6 hrs of anoxia (<0.05 mg 02 L-1) prior to infection by zoospores of P. cinnamomi. Roots were harvested and peroxidase activity separated into soluble, membrane, ionically bound and cell wall fractions. The majority of peroxidase activity was found in the fraction ionically bound to the cell wall. Peroxidase activity was increased within the lesion, but decreased elsewhere in infected roots compared to the uninfected controls. Roots exposed to prolonged hypoxia had the smallest lesions and lowest peroxidase activity. Thus, peroxidase activity per se is not responsible for the containment of lesions in infected roots.
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