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Genome level targeted breeding in wheat

Cakir, M., McLean, R., Wilson, R., Barclay, I., Moore, C., Loughman, R., Haley, S., Kidwell, K., Anderson, J., Sorrells, M., Manes, Y., Hayden, M., Feuillet, C., William, M., Hunter, A. and Bellgard, M. (2007) Genome level targeted breeding in wheat. In: Plant & Animal Genomes XV Conference, 13 - 17 January 2007, San Diego, CA


Molecular marker technologies offer a range of opportunities to plant breeders to improve the efficiency of breeding programs. The deployment of multiple genes relevant to a given environment is the major aim of a breeding program. To achieve this we describe a common approach and platform for MAS in wheat breeding programs, which we called this ‘genome level targeted breeding’. This approach would allow simultaneous selection of multiple target loci. The first aim is to establish a wheat map that includes important genes used in the breeding programs. The second is to maximize multiplexing ability for the marker screenings to be able to select for multiple traits, and the third is to discuss possible packaging option of the markers for specific breeding goals. The trait-based map may also serve a template to International Wheat Genome Sequencing Consortium for prioritising chromosomes and genomic regions while conducting physical mapping and eventually sequencing of wheat genome. Thus far, in collaboration with eight breeding programs including the CIMMYT, we have assembled a wheat map with the genomic locations of 85 genes. Additional breeding programs are expected to contribute to this process. For an initial study we have multiplexed 31 SSR markers for 22 traits and we have achieved this with having run each sample with two capillaries. We will describe and discuss various multiplexing options for specific breeding goals, different genotyping platforms, the use of bioinformatics tools for the generation of haplotype signatures for each trait and selection of lines for the breeders. Potential impact of the genome level targeted breeding on the breeding strategies will also be discussed.

Publication Type: Conference Paper
Murdoch Affiliation: Centre for Comparative Genomics
Western Australian State Agricultural Biotechnology Centre
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