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Measuring electrolytes: An evaluation of the IDEXX Vetlyte ion selective electrode method

Kavanagh, K. and Mills, J.N. (1997) Measuring electrolytes: An evaluation of the IDEXX Vetlyte ion selective electrode method. Australian Veterinary Practitioner, 27 (2). pp. 97-102.

Abstract

This study was conducted using more than 100 samples from three animal species (dog, cat and horse) to examine the differences in plasma sodium (Na), potassium (K) and chloride (Cl) readings made by the IDEXX Vetlyte ion selective electrode (ISE) methodology compared to flame photometry and chloridometry. The ISE measurements were significantly higher (P<0.001) by an average of 5.7%. The average differences were: Na, 8.2mmol/l; K, 0.21mmol/l and Cl, 6.9mmol/l. K values were most highly correlated by both methods in all species, and Cl in the horse showed the lowest correlation. Na values in the dog were better correlated than in the horse and cat. The precision of the ISE was acceptable, but with Na measurements showing more variation on the ISE than the flame photometer in between-run analyses. Reference ranges were determined for the ISE and were found to be higher than those available from the manufacturer for most electrolytes in the dog and cat, but the ranges were narrower for the horse. The presence of lipaemia in four plasma samples resulted in lower than average values for Na and Cl, but not K, when compared to nonlipaemic samples.

Publication Type: Journal Article
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Publisher: Australian Small Animal Veterinary Association
URI: http://researchrepository.murdoch.edu.au/id/eprint/18752
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