Subtractive hybridization of cDNA from small amounts of plant tissue
Heinrich, T., Washer, S., Marshall, J., Jones, M.G.K. and Potter, R.H. (1997) Subtractive hybridization of cDNA from small amounts of plant tissue. Molecular Biotechnology, 8 (1). pp. 7-12.
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A subtractive hybridization method is described that allows the generation of a subtractive gene library from small amounts of plant or other eukaryotic tissues. The method uses paramagnetic oligo-dT beads to capture poly-adenylated mRNA and to synthesize the complementary cDNA on a solid support. The use of magnetic beads facilitates the change of reaction buffers and the removal of primers and minimizes yield losses. Subtracted material obtained from this method can either be cloned directly or used to screen a specific library.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Western Australian State Agricultural Biotechnology Centre|
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