Differential effects of gadolinium chloride on Kupffer cells in vivo and in vitro
Lee, C.M., Yeoh, G.C. and Olynyk, J.K. (2004) Differential effects of gadolinium chloride on Kupffer cells in vivo and in vitro. The International Journal of Biochemistry & Cell Biology, 36 (3). pp. 481-488.
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Gadolinium chloride (GdCl) is commonly used to study the role of Kupffer cells in liver disease in vivo. The in vitro effects of GdCl on cultured Kupffer cells are poorly characterised. The aim of this study was to characterise rat Kupffer cell TNFα production, phagocytic function, and ED1 and ED2 antigen expression following the administration of GdCl. For in vivo experiments, rats received 10 mg/kg GdCl IV or sterile saline. Lipopolysaccharide 3 mg/kg IP (LPS) was administered 4 h prior to sacrifice on Days 1–3, 5 or 8 following GdCl injection. Hepatic ED1 and ED2 positive macrophage numbers and TNFα mRNA levels were determined. For in vitro experiments, Kupffer cells were cultured in the presence of 0–270 μM GdCl for 24 h following which viability, TNFα protein production in response to LPS (10 ng/ml), phagocytosis, and ED1 and ED2 staining were evaluated. In vivo, the proportion of ED1 positive cells which were ED2 positive was reduced from 87 to 3% and hepatic TNFα mRNA levels following LPS declined by 60% over Days 1–5 after injection of GdCl (P<0.01). In vitro, phagocytosis declined with increasing concentrations of GdCl. GdCl (0–27 μM) did not effect cultured Kupffer cell viability, TNFα production, ED1 or ED2 staining. We conclude that GdCl significantly reduces ED2 expression by Kupffer cells in vivo. In vitro, GdCl has a dose dependent effect on phagocytosis but only effects viability and TNFα production at high concentrations. ED2 expression of cultured Kupffer cells is not affected by GdCl.
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|Copyright:||© 2003 Elsevier Ltd.|
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