Quantitative PIXE and PIGME analysis of milligram samples of biomineralized tissue in the limpet Patella vulgata
Kim, K.S., Webb, J., Macey, D.J. and Cohen, D.D. (1987) Quantitative PIXE and PIGME analysis of milligram samples of biomineralized tissue in the limpet Patella vulgata. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 22 (1-3). pp. 227-230.
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Procedures have been developed to determine, by thick target PIXE and PIGME, the quantitative elemental composition of biological samples with a mass of approximately 1 mg. Systems of particular interest are the biomineralized tissues of chitons and limpets, marine invertebrates of global distribution whose radula teeth and associated tissue contain, variously, inorganic components at different stages of mineralization, e.g. Fe, Ca, P, F, Si, Cu. For the biomineralized teeth and tissue in the limpet Patella vulgata the content of Fe, Ca and P increases rapidly at an early stage of mineralization, while the Si content increases somewhat later. In fully mineralized teeth, the Fe and Si contents are comparable. These data are compared with previous results (Trends Biochem. Sci. 10 (1985) 6) obtained using the Oxford scanning proton microprobe.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Mathematical and Physical Sciences|
School of Environmental and Life Sciences
|Publisher:||North-Holland Publ Co|
|Copyright:||(c) North-Holland Publ Co|
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