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β-Thalassaemia/haemoglobin E tissue ferritins - II: A comparison of heart and pancreas ferritins with those of liver and spleen

Tran, K.C., Webb, J., Macey, D.J. and Pootrakul, P. (1990) β-Thalassaemia/haemoglobin E tissue ferritins - II: A comparison of heart and pancreas ferritins with those of liver and spleen. Biology of Metals, 3 (3-4). pp. 227-231.

Link to Published Version: http://dx.doi.org/10.1007/BF01140584
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Abstract

Tissue ferritins from β-thalassaemia/haemoglobin E heart and pancreas were characterized by native PAGE, SDS/PAGE and isoelectric focussing, and compared with those isolated from corresponding liver and spleen tissue. On PAGE; all ferritins consisted of a single band assigned to the protein monomer. Small differences in electrophoretic mobility were found between the bands. The ferritins were resolved by SDS/PAGE into two major subunits, H and L, corresponding to molecular masses of 22.5 kDa and 19 kDa, respectively. The L subunit was predominant in all cases. The isoferritin profiles of all tissue ferritins were remarkably similar; consisting of a complex pattern of bands which were appreciably more basic than those obtained for horse spleen ferritin. The subunit composition and isoferritin profiles of the four tissue ferritins almost certainly reflect the defense mechanism of the body in synthesizing in all four tissue types a more stable long-term iron-storage isoferritin in order to detoxify and store the excess iron present due to the pathological condition of β-thalassaemia/HbE.

Publication Type: Journal Article
Murdoch Affiliation: School of Biological and Environmental Sciences
Publisher: Springer-Verlag
Copyright: © 1990 Springer-Verlag
URI: http://researchrepository.murdoch.edu.au/id/eprint/1821
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