The proinflammatory and chondral activities of leukemia inhibitory factor in goat joints are partially a function of interleukin-1
Bell, M.C., Carroll, G.J., Chapman, H.M., Mills, J.N., Layton, M.J. and Saklatvala, J. (1999) The proinflammatory and chondral activities of leukemia inhibitory factor in goat joints are partially a function of interleukin-1. Journal of Interferon & Cytokine Research, 19 (2). pp. 197-208.
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We wished to determine if the effects of injected recombinant human leukemia inhibitory factor (LIF) are a function of endogenous goat interleukin-1 (IL-1) production and, conversely, if the effects of injected recombinant human IL-1 are a function of endogenous LIF production in goat radiocarpal joints (RCJ). In preliminary experiments, murine LIF binding protein (MuLBP) and recombinant HuIL-1RA were found to independently attenuate the cartilage proteoglycan resorbing activity of goat synovial membrane-conditioned medium (GSMCM), implying activity against goat LIF and goat IL-1, respectively. The present study shows that the proinflammatory and chondral actions of rHuLIF in goat RCJ are partially attenuated by rHuIL- 1RA. This implies that a small but important component of the in vivo activity of rHuLIF iS a result of IL-1 production in the synovial joint. With the exception of proteoglycan synthesis, the absence of significant effects by MuLBP on the actions of rHuIL-1α in goat RCJ suggests that the proinflammatory and chondral effects of IL-1α in vivo are probably not mediated by LIF.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary and Biomedical Sciences|
|Publisher:||Mary Ann Liebert Inc.|
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