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Towards developing transgenic resistance to nematodes in wheat

Jones, M.G.K., Rana, J., Fosu-Nyarko, J., Zhang, J., Perera, M.R. and Chamberlain, D. (2009) Towards developing transgenic resistance to nematodes in wheat. In: Riley, I.T., Nicol, J.M. and Dababat, A.A., (eds.) Cereal cyst nematodes: Status, research and outlook. Proceedings of the first Workshop of the International Cereal Cyst Nematode Initiative, 21-23 October 2009, Antalya, Turkey. CIMMYT, Ankara, Turkey, pp. 191-194.

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Research is in progress to use biotechnological tools to aid development of new forms of diagnostics and resistance to nematodes in wheat. For diagnostics, protein profiling by MALDI-TOF mass spectroscopy has been used to identify a range of nematode species, including seed gall, root-lesion and stem nematodes. Nematode species, and in some cases, races, can be identified and differentiated on the basis of characteristic protein biomarkers. A reproducible assay system for compounds that affect root-lesion nematode replication, based on carrot mini-discs, has been developed. Genomic studies have also been initiated to generate eDNA libraries and genomic sequences of root lesion and cereal cyst-nematodes. For new forms of genetic resistance to nematode pests of cereals, molecular approaches are in progress to identify new gene targets, generation of potential resistance-conferring gene constructs and their introduction into wheat by particle bombardment followed by regeneration and challenge to identify nematode resistant wheat plants.

Publication Type: Book Chapter
Murdoch Affiliation: Western Australian State Agricultural Biotechnology Centre
Publisher: CIMMYT
Copyright: © International Maize and Wheat Improvement Centre (CIMMYT) 2009
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