Catalog Home Page

The fhu genes of Rhizobium leguminosarum, specifying siderophore uptake proteins: fhuDCB are adjacent to a pseudogene version of fhuA

Stevens, J.B., Carter, R.A., Hussain, H., Carson, K.C., Dilworth, M.J. and Johnston, A.W.B. (1999) The fhu genes of Rhizobium leguminosarum, specifying siderophore uptake proteins: fhuDCB are adjacent to a pseudogene version of fhuA. Microbiology, 145 (3). pp. 593-601.

Free to read: http://dx.doi.org/10.1099/13500872-145-3-593
*No subscription required

Abstract

A mutant of Rhizobium leguminosarum was isolated which fails to take up the siderophore vicibactin. The mutation is in a homologue of fhuB, which in Escherichia coli specifies an inner-membrane protein of the ferric hydroxamate uptake system. In Rhizobium, fhuB is in an operon fhuDCB, which specifies the cytoplasmic membrane and periplasmic proteins involved in siderophore uptake, fhuDCB mutants make vicibactin when grown in Fe concentrations that inhibit its production in the wild-type. Nodules on peas induced by fhuDCB mutants were apparently normal in N2 fixation. Transcription of an fhuDCB-lacZ fusion was Fe-regulated, being approximately 10-fold higher in Fe-depleted cells. Downstream of fhuB, in the opposite orientation, is a version of fhuA whose homologues in other bacteria specify hydroxamate outer-membrane receptors. This fhuA gene appears to be a pseudogene with stop codons and undetectable expression.

Publication Type: Journal Article
Murdoch Affiliation: Centre for Rhizobium Studies
School of Biological Sciences and Biotechnology
Publisher: Society for General Microbiology
URI: http://researchrepository.murdoch.edu.au/id/eprint/17710
Item Control Page Item Control Page