Catalog Home Page

Wheat storage proteins in transgenic rice endosperm

Oszvald, M., Balázs, G., Pólya, S., Tömösközi, S., Appels, R., Békés, F. and Tamás, L. (2013) Wheat storage proteins in transgenic rice endosperm. Journal of Agricultural and Food Chemistry, 61 (31). pp. 7606-7614.

Link to Published Version:
*Subscription may be required


Transgenic rice seed expressing wheat HMW glutenin subunit was characterized to study the effects of the wheat prolamin on the protein expression pattern and protein size distribution in the endosperm and the functional and rheological properties of the rice flour and dough. Significant differences were found in the protein expression pattern between the transgenic and wild type samples. Comparing the protein expression profiles of transgenic and nontransgenic plants, combined with proteomic-based studies, indicated increased protein disulfide isomerase (PDI) levels in the transgenic rice lines. The accurate molecular size of HMW-GS in rice endosperm was identified by MALDI-TOF-MS analysis. The expressed wheat HMW (subunit 1Dx5) GS showed a positive effect on the functional properties of rice dough by significantly increasing the size distribution of the polymeric protein fraction and modifying the dough mixing parameters.

Publication Type: Journal Article
Murdoch Affiliation: Centre for Comparative Genomics
Publisher: American Chemical Society
Copyright: © 2013 American Chemical Society
Item Control Page Item Control Page