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Interpreting sub-optimal nitrogen fixation in Lupinus angustifolius utilising the genome sequence of both symbiotic partners

Bellgard, M., Reeve, W., Yang, H., Zhang, J., Barrero, R. and Howieson, J. (2011) Interpreting sub-optimal nitrogen fixation in Lupinus angustifolius utilising the genome sequence of both symbiotic partners. In: 17th International Congress on Nitrogen Fixation, 27 November - 1 December 2011, Fremantle, WA.

Abstract

WSM1417, WSM1253 and WSM471 are nodule bacteria that infect Lupinus angustifolius (NLL narrow-leaf lupin) and which have different nodulation and nitrogen fixation profiles. These three strains were sequenced within the JGI GEBA project through the US Department of Energy. To understand the genetic control of the relationship between these three micro-symbionts and their legume host we required both the lupin genome sequence, and transcriptome information. We thus sequenced NLL cv Tanjil using a WGS approach and achieved a 30 fold coverage of the whole genome. We obtained 31 billion base pair high quality sequencing data and are undertaking the lupin genome assembly and annotation with a view to identify candidate genes in nodule formation and function. The transcriptome data was of nodules of three NLL plants inoculated with the three sequenced strains. From the NLL genome sequence we obtained 23,000 contigs, which were assembled into 12,500 scaffolds. The largest contig we have is 149Mb, the N50 size of 13.6 Kb and there is ~80Mb of repeat sequences. Further analysis of the genome sequence data will be provided.

Publication Type: Conference Item
Murdoch Affiliation: Centre for Comparative Genomics
Centre for Rhizobium Studies
School of Biological Sciences and Biotechnology
URI: http://researchrepository.murdoch.edu.au/id/eprint/17404
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