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Preterm infant IFN-Beta responses to Group B streptococcus

Ng, Sherrianne (2013) Preterm infant IFN-Beta responses to Group B streptococcus. Honours thesis, Murdoch University.

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Group B streptococcus (GBS) is the leading cause of early onset sepsis in preterm infants. The production of IFN-Beta shown to be critical for host defense against GBS in murine studies. The release of GBS-DNA into the cytosol of mouse macrophages was found to induce IFN-Beta monocytes and macrophages produce IFN-Beta GBS-DNA plays in this process. This study optimised a whole blood assay to determine if human monocyte subsets are capable of inducing IFN-Beta as cytosolic GBS-DNA. The main findings were that (A) all adult blood monocyte subsets were capable of producing IFN-Beta cytosolic GBS-DNA, as determined by IP-10 expression, (B) the intermediate monocyte subset produced the highest amount of IP-10 to both live GBS and cytosolic GBS-DNA, and (C) the monocytes of a preterm infant had similar IP- 10 responses to cytosolic GBS-DNA but much lower IP-10 responses to live GBS compared to adults. These findings indicate that human blood monocytes are capable of producing IFN-

Publication Type: Thesis (Honours)
Murdoch Affiliation: School of Veterinary and Life Sciences
Supervisor: Currie, Andrew and Townsend, Kirsty
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