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Sonication as an aid for handling geraldton wax (Chamelaucium uncinatum) pollen

Newell, C., McComb, J. and Lullfitz, G. (1998) Sonication as an aid for handling geraldton wax (Chamelaucium uncinatum) pollen. Acta Horticulturae, 454 . pp. 365-371.

Link to Published Version: http://www.actahort.org/books/454/454_43.htm
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Abstract

Pollen in Chamelaucium species is exuded from the anthers in an oily substance and deposited onto the pollen presenter. Pollen in oil was sonicated in 20% sucrose at 20 watts, then spun down at 8,000 rpm for 60 s. Pollen germination was reduced by sonication (from 50% to 30%), and pollen tube length was reduced by about one third in 2 of the 3 lines tested. When sonicated pollen was used on the stigma, good germination was observed; by 48 h there were as many pollen tubes at the base of the style as when using unsonicated pollen. Overall 11.5% seed set was observed using sonicated pollen in intraspecific crosses of C. uncinatum, and 7% in C. uncinatum x megalopetalum crosses.

Publication Type: Journal Article
Murdoch Affiliation: School of Biological and Environmental Sciences
Publisher: International Society for Horticultural Science
URI: http://researchrepository.murdoch.edu.au/id/eprint/16097
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